In the above example, both fluorescent stainings to the left and in the middle show a similar pattern of location. The image of overlay (to the right) is little informative as for the colocalization of both markers. An analysis of colocalization by segmentation leads to obtain this quantification. A wavelet segmentation is applied to every channel to detect the fluorescent objects. This segmentation is made in 3D to increase precision. The measure of colocalization is defined as the ratio of pixels segmented in both channels. While the analysis of the left images and the center images gives a feeling of an important level of colocalization, the quantitative analysis reveals a very low level (5,6 %). The quantitative analysis thus allows to avoid the important errors of the visual interpretation.