Automatic detection of the growth of axons in microscopy of fluorescence

An explant of the medulla is cultivated in the presence of a chemical attractor. Directionality of the axon growth is studied. In the following illustration, the explant is labeled in GFP alpha-tubulin. Axons outside of the explant are automatically detected. Their direction is represented in the rose diagram, showing the influence of the chemical attractor, in a statistical, reproducible and unbiased manner.